Co-immobilization effect on H2 production by a chloroplast membranes-hydrogenase system

Abstract

Chloroplast membranes immobilized within a BSA-GA matrix or within an alginate gel have been associated with native or immobilized hydrogenase in order to produce hydrogen gas through biophotolysis of water. Due to the reaction geometry, co-immobilization of chloroplast membranes with the enzyme inside the same matrix considerably improved the amount of H2 produced and the initial activity. The use of entrapment methods such as alginate gel allowed diffusion of proteins through the matrix. Electron microscopic observations illustrated these results.