Abstract
Bacillus coagulans is an interesting facultative anaerobic microorganism for biotechnological production of lactic acid that arouses interest. To determine the efficiency of biotechnological production of lactic acid from lignocellulosic feedstock hydrolysates, five Bacillus coagulans strains were grown in lignocellulose organosolv hydrolysate from ethanol/water-pulped beechwood. Parameter estimation based on a Monod-type model was used to derive the basic key parameters for a performance evaluation of the batch process. Three of the Bacillus coagulans strains, including DSM No. 2314, were able to produce lactate, primarily via uptake of glucose and xylose. Two other strains were identified as having the ability of utilizing cellobiose to a high degree, but they also had a lower affinity to xylose. The lactate yield concentration varied from 79.4 ± 2.1 g/L to 93.7 ± 1.4 g/L (85.4 ± 4.7 % of consumed carbohydrates) from the diluted organosolv hydrolysate.
Highlights
Renewable biomass has been demonstrated to be suitable for the biotechnological production of biofuels and basic chemicals
Batch fermentation processes were performed on lignocellulose organosolv hydrolysate
The hydrolysate was obtained from the second organosolv fractionation procedure conducted in the pilot plant by the Fraunhofer Centre for Chemical Biotechnological Processes CBP in Leuna, Germany
Summary
Renewable biomass has been demonstrated to be suitable for the biotechnological production of biofuels and basic chemicals. Such biomass can be starchy biomass, sugar-based feedstocks, and lignocellulosic biomass [1]. Several pre-treatment methods exist to hydrolyse the bound carbohydrates – such as glucose, xylose, arabinose, galactose, and mannose – for fermentation. The pre-treatment methods that can be used to break down the structure of lignocellulosic biomass are categorized into five groups; i.e. physical treatment (e.g., mechanical disruption), chemical treatment (e.g., alkali, dilute acid, organosolv), thermal treatment (e.g., steam explosion), physicochemical treatment (e.g., ammonia fibre explosion, AFEX) and biological treatment (e.g., degradation by enzymes) [7,8,9,10]
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