Abstract
Purpose: Worldwide Gastric carcinoma considered as the second most common cause of cancer related death. Cancer stem cell plays significant role in prognosis and invasion of gastric cancer. CXCR4 is a chemokine receptor and plays an important role in self renewal, differentiation potential, and cell adhesion of cancer stem cell (CSC). On the other hand CD133 is a cell surface glycoprotein and could serve as a prognostic indicator for tumor re-growth, malignant progression, and patient survival. The aim of this study was to establish the expression pattern of CXCR4 and CD133 in gastric cancer tissues; and their correlation with clinicopathological factors like age and gender of patients, position, size and depth of tumor, lymphatic invasion and node metastasis. Methods: Expression of CXCR4 and CD133 proteins were assessed by immunohistochemical and immunofluorescence staining of paraffin–embedded tissues, and followed by RT-PCR in 90 tumors (observed group) and 30 normal gastric samples. The clinical pathological data was statistically analyzed by chi-square methods. Results: The positive rate of CXCR4 and CD133 expression in the observed group was 94.44 (85/90) and 95.55 (86/90) respectively. The expression of CXCR4 and CD133 were correlated with age and gender of patients, and position, size, & depth of the tumor, lymphatic invasion and node metastasis ( p < 0.05). While CXCR4 was positive, CD133 had a positive rate of 92.22% but the positive rate was 2.22% when CXCR4 expression was negative (χ 2 = 58.657; p < 0.001). Conclusion: Overall this data suggests that increased expression of CXCR4 and CD133 might be attributed with disease progression and malignant transformation of gastric epithelium cells. A significant correlation was found in between CXCR4 and CD133 expression and their co-expression may play significant role in invasiveness of gastric cancer.
Highlights
Gastric cancer (GC) is one of the commonest forms of malignancies in the world
The expression of CXCR4 and CD133 in gastric cancer and adjacent normal gastric mucosa IHC assay showed that CXCR4 positive staining mainly localized on cell cytoplasm (Figure 1A), and brownish granule of CD133-positive were visible in cytoplasm and the cell membrane (Figure 1B), which both didn’t express in gastric normal tissues
The positive rate of CXCR4 expression was 85/90 (94.44%) and 7/30 (23.23%) in gastric cancer and adjacent normal tissues, with statistically significant differences (p
Summary
Gastric cancer (GC) is one of the commonest forms of malignancies in the world. Pathogenesis of gastric cancer is associated with activation of oncogenes, inactivation of tumor suppressor genes and deregulation of cellular signal pathways. The gastric epithelial cells undergo stepwise morphologic changes before malignant transformation and multiple signaling pathways have been known to be involved in this carcinogenic process.
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