Abstract
ABSTRACT Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in S. cerevisiae to enhance the practical biotransformation, two recombinant Saccharomyces cerevisiae strains (INVSc1-CBH-CA and INVSc1-CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in INVSc1-CBH-CA and INVSc1-CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/mL, respectively. The recombinant S. cerevisiae can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than S. cerevisiae only expressing cellulase. Fermentation with INVSc1-CBH-CA and INVSc1-CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than INVSc1-CBH, 2.8 and 3.4 folds higher than the wild type S. cerevisiae. The strategy of co-expression cellulase and xylanase in saccharomyces cerevisiae is effective and can be a foundation to research the mechanism of synergy effect of cellulose and xylanase.
Highlights
Lignocellulose is a major constituent of plant biomass and consists mainly of cellulose, hemicellulose, and lignin etc [1,2]
The mixed linearized plasmids were electrotransformed into S. cerevisiae INVSc1 and screened by dual auxotrophic screening medium SCUra-Leu plates to obtain recombinant strains coexpressing cellulases and xylanases
The results showed that the cellulase and xylanase genes were successfully co-expressed in S. cerevisiae
Summary
Lignocellulose is a major constituent of plant biomass and consists mainly of cellulose, hemicellulose, and lignin etc [1,2]. Some researchers statistical analysis the related parameters during bioethanol production and build modeling. They found in most of bioethanol production process the residues cannot be completely utilized for energy recovery. The cellulose and hemicellulose in lignocellulose are hydrolyzed to obtain pentose and hexose, which can be used as raw materials [7,8]. Lignin is another major component in lignocellulose which increases the material recalcitrant and the difficulty of enzymolysis [9]. In the presence of such synergies, cellulose degradation is more efficient
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
