Co‐compartmentalization of Enzymes and Cofactors within Pickering Emulsion Droplets for Continuous‐Flow Catalysis

Abstract

AbstractCo‐immobilization of enzymes and cofactors in a manner suitable for use in continuous flow catalysis remains a great challenge because of the difficulty in ensuring the free accessibility of immobilized enzymes and cofactors. Herein, we present a continuous flow catalysis system based on co‐compartmentalization of enzymes and cofactors within Pickering emulsion droplets, enabling regeneration of cofactors within the droplets. As exemplified by enzyme‐catalyzed ketone enantioselective reduction and enantioselective transamination, our systems exhibit long‐term stability (300–400 h), outstanding total turnover number (TTN, 59204 mol mol−1) and several‐fold enhancement in the enzyme catalytic efficiency (CEe) in comparison to conventional biphasic reactions. As well as giving insight into the co‐compartmentalization effects, our system will provide the opportunity to significantly advance continuous‐flow biocatalysis towards the level of practical applications.