Abstract
Between October 2001 and April 2002, five cases of acute flaccid paralysis (AFP) associated with type 2 vaccine-derived polioviruses (VDPVs) were reported in the southern province of the Republic of Madagascar. To determine viral factors that favor the emergence of these pathogenic VDPVs, we analyzed in detail their genomic and phenotypic characteristics and compared them with co-circulating enteroviruses. These VDPVs appeared to belong to two independent recombinant lineages with sequences from the type 2 strain of the oral poliovaccine (OPV) in the 5′-half of the genome and sequences derived from unidentified species C enteroviruses (HEV-C) in the 3′-half. VDPV strains showed characteristics similar to those of wild neurovirulent viruses including neurovirulence in poliovirus-receptor transgenic mice. We looked for other VDPVs and for circulating enteroviruses in 316 stools collected from healthy children living in the small area where most of the AFP cases occurred. We found vaccine PVs, two VDPVs similar to those found in AFP cases, some echoviruses, and above all, many serotypes of coxsackie A viruses belonging to HEV-C, with substantial genetic diversity. Several coxsackie viruses A17 and A13 carried nucleotide sequences closely related to the 2C and the 3Dpol coding regions of the VDPVs, respectively. There was also evidence of multiple genetic recombination events among the HEV-C resulting in numerous recombinant genotypes. This indicates that co-circulation of HEV-C and OPV strains is associated with evolution by recombination, resulting in unexpectedly extensive viral diversity in small human populations in some tropical regions. This probably contributed to the emergence of recombinant VDPVs. These findings give further insight into viral ecosystems and the evolutionary processes that shape viral biodiversity.
Highlights
Polioviruses (PVs), members of the Enterovirus genus in the Picornaviridae family, are major human pathogens causing the acute paralytic disease poliomyelitis
Several poliomyelitis outbreaks associated with vaccine-derived polioviruses (VDPVs) were reported in different parts of the world in recent years, in Madagascar in 2002
Similar VDPVs and other enteroviruses, including several human enteroviruses of species C (HEV-C) of different types, were found in the stools of healthy children living in neighboring villages to where most of the poliomyelitis cases occurred
Summary
Polioviruses (PVs), members of the Enterovirus genus in the Picornaviridae family, are major human pathogens causing the acute paralytic disease poliomyelitis. The human enteroviruses (HEV) are classified into five species, HEV-A to -D, and the PV species (PV-1 to À3). The species HEV-C includes several serotypes of coxsackie A virus, and segregates in the same phylogenetic cluster (cluster C) as the PV species [1]. Enteroviruses, including PVs, are small non-enveloped viruses with a positive-strand RNA genome about 7.5 kb long. The single large coding region of the genome is flanked by 59- and 39-UTR. The coding region is translated as a single polyprotein that is processed by viral proteases to yield the mature viral proteins including the capsid proteins VP1 to VP4 and non-structural proteins including proteases and the RNA-dependent RNA polymerase 3Dpol
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