Co- and Counter-Electroosmotic Flow Capillary Electrophoresis of Anionic Phenols Using UV and MS Detection

Abstract

Co- and counter-electroosmotic flow (EOF) capillary electrophoresis (CE) of anionic phenols, using UV and mass spectrometry (MS) detection were investigated. The co-EOF CE-UV detection was performed by using hexadimethrine bromide (HDB) as an EOF modifier, which provided the separation in 6.2 min. The counter-EOF CE-MS was obtained without HDB and coupling was achieved by sheath liquid interface. The CE-UV provided the better linearity (r 2 > 0.99) than the CE-MS (r 2 > 0.81), however, both methods gave comparable detection limits (5 µg/mL). CE-UV is efficient for the routine quantitative analysis, whereas CE-MS is useful for identification of the phenols.