Abstract
CO2 accumulation in different culture systems containing embryogenic cell suspension cultures of cyclamen (Cyclamen persicum Mill.) was analyzed. In bioreactors equipped with a bubble-free or a bubble aeration system, CO2 mole fractions in the gas phase of more than 10% were determined whereas in Erlenmeyer flasks, CO2 mole fractions were below 2%. CO2 accumulation in bioreactors was severely growth inhibiting in comparison to the flasks. By removing CO2 in the aeration gas of a bubble-free aerated bioreactor, cell growth comparable to that in flasks was achieved. The regeneration ability of cell suspensions after being cultured in bioreactors with CO2 accumulation was better than those after culture in bioreactors without CO2 accumulation or in flasks.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
