CNSC-34. BAF60C/SMARCD3 MODULATES CEREBELLUM DEVELOPMENT AND MEDULLOBLASTOMA METASTASIS

Abstract

Abstract Medulloblastoma (MB), the most common type of embryonal tumor in children, is a fast-growing and heterogeneous brain tumor arising in the cerebellum. The molecular characterization of MB reveals four major subgroups (WNT, SHH, Group 3, and Group 4) and Group 3 is the most aggressive and malignant, characterized by frequent metastasis at diagnosis and the worst prognosis. We have recently found that BAF60C/SMARCD3 is highly expressed in Group 3 MB and associated with poor prognosis. Mechanistically, BAF60C/SMARCD3 hijacks a neurodevelopmental epigenetic program mediated by Disabled1 (DAB1)-Reelin signaling to promote metastatic dissemination in MB. Using single-cell RNA sequencing data of developing mouse and human cerebellum, we found that BAF60C/SMARCD3 expression in Purkinje cells (PCs) is upregulated during embryonic and early postnatal stages, but downregulated during the late postnatal stage of cerebellar development. To examine the effect of BAF60C/SMARCD3 depletion on PC migration and positioning during embryonic and postnatal stages of cerebellar development, we generated targeted knockout mice by crossing the PC-specific Cre mouse (L7Cre-2 and FoxP2-Cre) with the Smarcd3flox/flox mouse. We found that BAF60C/SMARCD3 depletion significantly influences embryo development in FoxP2-Cre; Smarcd3flox/flox but not in L7Cre-2; Smarcd3flox/flox mice, further supporting the expression of BAF60C/SMARCD3 in early stage (Foxp2 +) but not in the late stage (L7/Pcp2+) during cerebellum development. To determine whether BAF60C/SMARCD3 contributes to MB development, we injected lentivirus-carrying constitutively active MYCS62D with/without BAF60C/SMARCD3 in C57BL/6J mice. Interestingly, we found that MYCS62D with BAF60C/SMARCD3 can induce a spontaneous Group3-like MB with strong metastatic dissemination compared to MYCS62D without BAF60C/SMARCD3. These results demonstrate that BAF60C/SMARCD3 plays a pivotal role in PC biology and cerebellar development, and tumor cells hijack BAF60C/SMARCD3-Reelin signaling to promote tumor metastasis in MB.