Abstract
This experiment was carried out to select resistant cultivar to CMV in cucumber using Elisa-test and protoplast isolation method. Twenty domestic cultivars or lines and 8 European cultivars were ested for resistance by Elisa test. Among the domestic cultivars, DADAKI group was found to be susceptible and CHEONGJANG group resistant. Among all the cultivars and lines tested, a European cultivar, DALIBOR and Janghyungheukjinju Korean line were found to be highly resistant. When comparing for the protoplast yield depending upon the positions of seedlings (cotyledon, young leaf and hypocotyl), the highest protoplast yield could be obtained from cotyledons in macerozyme 0.5% + cellulose 2.0%. Protoplast yields in susceptible cultivars were higher than those from resistant cultivars. Differences in cell wall thickness between susceptible and resistant cultivars were observed.
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