Abstract
The quality of the ethanol extract of red betel leaves as a medicinal plant is determined by secondary metabolites, which are influenced by geographical conditions and the plant growing environment. This study aims to classify red betel from various regions in Indonesia based on secondary metabolite fingerprint analysis and cytotoxicity values. Observation of the diversity of secondary metabolites of the ethanol extract of red betel leaves from seven different regions (Banda Aceh, Bandung, Bogor, Malang, Samarinda, Kendari, Jayapura) was carried out using a metabolomics approach using liquid chromatography-mass spectroscopy (LC-MS/MS) and determining cytotoxicity value using the Brine Shrimp Lethality Test (BSLT) method. Secondary metabolite fingerprinting analysis using cluster analysis with dendrogram yielded 12 compounds with 3 sample groups based on their region of origin, namely group 1 (Banda Aceh, Samarinda, Jayapura); group 2 (Bandung, Kendari, Malang); group 3 (Bogor). Group 1 samples identified eight compounds that had the highest relative abundance values. Group 2 samples identified 3 compounds that have the highest relative abundance values. Group 3 samples had 1 compound with the highest relative abundance value. Each compound has a different retention time. The cytotoxicity value (LC50) of the ethanol extract of betel leaves was obtained from the Banda Aceh and Malang areas (2.64 µg/mL). The conclusions of this study based on the results of secondary metabolite fingerprinting analysis and cytotoxicity values identified 12 compounds with three clusters of secondary metabolite diversity based on their region of origin, namely group 1 (Banda Aceh, Jayapura, Samarinda); group 2 (Bandung et al.); group 3 (Bogor).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.