Abstract
9024 Background: Ipilimumab (Ipi) is approved for metastatic melanoma based on significant survival benefits shown in phase III trials. Ipi can result in immune-mediated effects on various organ systems, leading to adverse events (AE) including diarrhea/colitis reported in 30-40% patients (pts) that can be serious and life-threatening. The ability to predict the risk of this AE may lead to new monitoring and improved management. Methods: Targeted, deep sequencing of DNA from the blood of 34 pts who underwent neoadjuvant Ipi for regionally advanced melanoma (Tarhini et al, PLOS One, 2014) was performed to detect the 21 pts who experienced diarrhea/colitis. Sequencing was focused on the coding region of 10 genes and genomic domains surrounding 9 SNPs previously associated with IBS, Crohn’s Disease and ulcerative colitis in GWAS studies. Selected amplicon libraries derived from locations on 5 chromosomes were sequenced on Ion Torrent PGM at high base density (318 chip: >1000X) and BAM files interrogated usin...
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