Clostridium thermobutyricum sp. nov., a Moderate Thermophile Isolated from a Cellulolytic Culture, That Produces Butyrate as the Major Product

Abstract

A new moderately thermophilic clostridium, Clostridium thermobutyricum, was isolated from cellulolytic enrichment cultures inoculated with horse manure. This organism forms subterminal spores, and the sporangium is not swollen. From glucose, the organism produces butyrate, CO2, and H2 and minor amounts of acetate and lactate. Yeast extract is required for good growth. The temperature range for growth is between 26 and 61.5°C. The pH range is between 5.8 and 9.0. The guanine-plus-cytosine content is 37 mol%. The type strain is C. thermobutyricum JW171K (= DSM 4928).