Abstract
In 2014, 18 hospitals in the Czech Republic participated in a survey of the incidence of Clostridium difficile infections (CDI) in the country. The mean CDI incidence was 6.1 (standard deviation (SD):7.2) cases per 10,000 patient bed-days and 37.8 cases (SD: 41.4) per 10,000 admissions. The mean CDI testing frequency was 39.5 tests (SD: 25.4) per 10,000 patient bed-days and 255.8 tests (SD: 164.0) per 10,000 admissions. A total of 774 C. difficile isolates were investigated, of which 225 (29%) belonged to PCR ribotype 176, and 184 isolates (24%) belonged to PCR ribotype 001. Multilocus variable-number tandem repeat analysis (MLVA) revealed 27 clonal complexes formed by 84% (190/225) of PCR ribotype 176 isolates, and 14 clonal complexes formed by 77% (141/184) of PCR ribotype 001 isolates. Clonal clusters of PCR ribotypes 176 and 001 were observed in 11 and 7 hospitals, respectively. Our data demonstrate the spread of two C. difficile PCR ribotypes within 18 hospitals in the Czech Republic, stressing the importance of standardising CDI testing protocols and implementing mandatory CDI surveillance in the country.
Highlights
Clostridium difficile is the most important bacterial cause of hospital-acquired diarrhoea
Czech Republic had a low incidence of Clostridium difficile infections (CDI) (1.1/10,000 patient bed-days), without the presence of C. difficile
Mean CDI incidence and CDI testing frequency for all participating hospitals was calculated using the total number of admissions, total number of patient beddays, number of non-duplicated glutamate dehydrogenase (GDH) and toxin A/B positive tests performed in
Summary
Clostridium difficile is the most important bacterial cause of hospital-acquired diarrhoea. Two large studies have been carried out to map and update data on. C. difficile infection (CDI) in Europe [1,2]. CDI incidence showed an increasing trend: in the first study in 2008, the mean incidence in the participating countries was. 4.1 cases per 10,000 patient bed-days [1], while in the second, in 2011–13, it was 7.0 CDI cases per 10, 000 patient bed-days in the countries involved [2]
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