Abstract
Two strains of sporeforming, xylan-digesting, rod-shaped bacteria were isolated from a 10−8dilution of rumen ingesta taken from two sheep fed a corn stover ration. To determine the source of these strains, we examined the corn stover and isolated two strains of similar bacteria from it. The four strains were remarkably tolerant to oxygen although they did not grow in liquid medium that was shaken while exposed to air. They fermented a wide variety of carbon sources and produced formic, acetic, and lactic acids, ethanol, carbon dioxide, and hydrogen from xylan. The deoxyribonucleic acid base composition was 40 mol% guanine plus cytosine. The name proposed for these strains is Clostridium aerotolerans; the type strain is strain X8A62 (ATCC 43524).
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