Clostridium acetobutylicum CPCNE之氮源及添加劑利用研究

Abstract

The high butanol-producing strain named Clostridium acetobutylicum CPCNE was activated from the potential hyper-butanol-producing strain library of Clostridium stored in Refining and Manufacturing Research Institute of CPC Corporation in Taiwan. In this study, Clostridium acetobutylicum CPCNE was fermented with different concentrations of CSL (corn steep liquor, as nitrogen source) and additives in batch-scale flasks. 4% CSL was found to be the best feasible for the butanol production as nitrogen source. Compared to the traditional ABE fermentation producing more acetone than ethanol, ethanol was produced more than acetone when CSL was used as nitrogen source in the fermentation. CaCO3, FeSO4．7H2O and NaAc could be used to improve the yield of butanol, whereas the fermentation of Clostridium acetobutylicum CPCNE could slowed down and stopped by the addition of cysteine-HCl and butyric acid.The ABE fermentation in batch-scale could be finished within two days while 13.61 g/L butanol and 21.99 g/L ABE solvent were produced with ABE yield and productivity of 0.38 g/g and 0.46 g/L/h, respectively.