Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing.

Cedric Woudstra,Christelle Mazuet,Antti Honkela,Tommi Mäklin,Miia Lindström,Yagmur Derman,Hanna Skarin,Luca Bano,Irene L G Newton

doi:10.1128/mra.01364-20

Abstract

ABSTRACTClostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.

Highlights

Clostridium botulinum group III strains producing botulinum neurotoxin (BoNT) types C and D and their mosaic forms, C/D and D/C, are responsible for animal botulism (1)
This resulted in a characteristic white cloud precipitation containing HMW DNA
HMW DNA was pelleted by centrifugation at 3,500 rpm for 1 min at 4°C

Summary

Introduction

Clostridium botulinum group III strains producing botulinum neurotoxin (BoNT) types C and D and their mosaic forms, C/D and D/C, are responsible for animal botulism (1). Received 6 January 2021 Accepted 24 March 2021 Published 3 June 2021 mra.asm.org 1 PacBio No of Chromosome content sequences size (Mbp) (%) (PGAAP) PacBio SRA Illumina accession sequencing Illumina SRA

Results

Conclusion