Abstract
Photoacoustic imaging using a closed photoacoustic cell and an open photoacoustic cell with gas- microphone detection scheme was described. R/G/B LED light sources were used for the closed photoacoustic (PA) cell configuration. The colored specimen enclosed in a PA cell was imaged with R/G/B color light sources, and an image restored from the inverted PA images was compared with the original image. For open cell configuration, an open PA cell using a spheroidal acoustic resonator was applied to measure the amount of large-sized colored specimens. A calibration curve for a food red dye was obtained that apparently showed the ability of the present scheme to measure as a spectroscopic measurement tool.
Highlights
In the photoacoustic microscopy (PAM) or photoacoustic (PA) imaging, gas-microphone and piezo-electric detections have been used for the spectroscopic imaging or nondestructive evaluation (NDE) of specimens
One approach is to use multiple wavelengths light sources which can detect specimen color with the ability to measure its amount for objective i), and the other approach is to develop an effective coupling between PA detector and the specimen located outside the PA cell in order to measure a large-sized spectroscopic specimen for objective ii)
The original color restoration procedure was as follows: The gray-images obtained with R/G/B LEDs were colored with their complementary colors
Summary
In the photoacoustic microscopy (PAM) or photoacoustic (PA) imaging, gas-microphone and piezo-electric detections have been used for the spectroscopic imaging or nondestructive evaluation (NDE) of specimens. In the spectroscopic analysis with PA imaging, PA imaging apparatuses with monochromatic light sources have been used and were applied to measure a paper chromatography [1], dry chemical analysis [2] and pollen measurement [3] up to now These applications to imaging of microscale spectroscopic objects with PAM prefers a multiple-wavelength optical source rather than a monochromatic one and an ability to analyze large-size specimens is required. One approach is to use multiple wavelengths light sources which can detect specimen color with the ability to measure its amount for objective i), and the other approach is to develop an effective coupling between PA detector (microphone) and the specimen located outside the PA cell in order to measure a large-sized spectroscopic specimen for objective ii).
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