Abstract
Small samples of DNA can be replicated (amplified) using the polymerase chain reaction (PCR) to produce sufficient quantities for analysis in the diagnosis of disease and in forensics. Low-cost single-shot PCR devices are desirable to avoid potential contamination of the sample reaction with products of a previous reaction. After explaining the principles of PCR amplification and optical detection of the products this paper describes the fabrication of a micromachined silicon/Pyrex-based chip for the PCR using standard micromachining processes. The reaction chamber has a volume of 1 μl. Pyrex is used for sealing the chamber and its transparency enables the use of optical detection methods. Incorporated heating and sensing elements allow fast heating and control of the chip. A Peltier element is used for cooling the chip to achieve rapid temperature cycling. Some performance data are presented.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
