Clorofilina inibe a multiplicação de poliovírus em cultura de células hep-2

Abstract

Chlorophyllin (CHLN), a semisynthetic derivative of chlorophyll with antimutagenic properties, was assayed for its antiviral effect on the replication of poliovirus in cell culture. The drug was tested for the virucide, prophylactic and therapeutic activities on the replication of the poliovirus in HEp-2 cells cultures, at concentrations of 0.5 and 2.5 µg/mL. Virus titration and an indirect immunofluorescence test were used for the evaluation. The CHLN inhibited poliovirus replication in all treatment protocols; however, it was more effective on virucide treatment, with a 95.7% reduction in viral multiplication at concentration of 2.5 µg/mL. CHLN reduced the number of specifically fluorescent infected cells in both virucide and therapeutic treatments, 8h and 10h post-infection, at both concentrations (0.5 and 2.5 µg/ mL). It is suggested that CHLN either has a direct action on the virus particles or acts on the initial stage of the poliovirus replication.