Abstract
TheVgfgene was originally identified as a 2.7-kb cDNA fragment isolated from nerve growth factor-treated PC12 cells by differential display against PC12 cells. It is transcribed solely in subpopulations of neuroendocrine cellsin vivoand it is induced by neurotrophins in target cellsin vitro.The single-copy humanVGFgene was isolated from a genomic library. The gene spans approximately 6 kb and contains two exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5′-untranslated sequence. The structural organization of the human gene is similar to that described for the ratVgfgene (S. R. J. Saltonet al.,1991,Mol. Cell. Biol.11: 2335–2349) and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene. Northern blot analysis revealed a single transcript of approximately 2.7 kb that was detected only in mRNA preparations from brain. The gene was assigned to chromosome 7q22 by fluorescencein situhybridization.
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