Abstract
Leaves of the indigo plant ( Polygonum tinctorium) contain a β-glucosidase with a high activity for the substrate indican. The β-glucosidase cDNA was cloned from a leaf cDNA library. The cDNA consists of 1813 nucleotides including 1533 nucleotides of an open reading frame (ORF). The ORF in the cDNA encoded a total of 511 amino acids containing 483 amino acids of a mature protein and 28 of a transit peptide. Interestingly, an internal homologous region spanning the transit peptide and N-terminal sequence was found. The deduced amino acid sequence of the mature enzyme showed a high homology with that of β-glucosidases from several other plants and bacteria, and a well conserved region of several amino acids that forms the pocket of the active site. The cDNA was expressed in E. coli and the cell extract was confirmed to have β-glucosidase activity.
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