Abstract
AbstractTo amplify the transferrin-binding protein 2 (Tbp2) gene fromActinobacillus pleuropneumoniae(App) by polymerase chain reaction (PCR), a pair of primers were designed according to sequence Z46774 ofA. pleuropneumoniaeserotype 5. The amplified DNA fragment (1642 bp) was cloned into pMD18-T and sequenced. The sequencing result showed that the homology was 99.8% compared with the reference sequence. The target fragment of the positive clones was inserted into pET-32a and transformed inEscherichia coliBL21 (DE3). After its induction, the fragment was expressed inE. coli. The Western blotting results were positive.
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