Abstract
Using degenerate primers based on insulin sequences from other organisms, we report the cloning of the complete tilapia ( Oreochromis niloticus) insulin gene. Using nested primers and a cassette ligation strategy we have also cloned 932 base pairs (bp) of 5′ flanking and 1152 bp of 3′ flanking sequence. The tilapia insulin gene has the similar three exon (one untranslated), two intron distribution found in all insulin genes sequenced to date. However, intron 1 is unique in having a smaller size (73 bp) than found in other organisms. 5′ RNA extension revealed the presence of two potential transcriptional start sites. A perfect TATA box is located at −30 bp from the first transcriptional start site. Interestingly, the 5′ upstream region contains a microsatellite close to the same position of a unique minisatellite found only in humans and primates. The upstream region also contains several potential control elements to regulate insulin expression that are found in mammalian insulin genes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
