Abstract

Manganese (Mn) specific hyperaccumulation in the tree Chengiopanax sciadophylloides in soil not polluted by metals has received attention as a distinctive character of this plant, but its mechanism has not yet been clarified. Based on the speculation that ZIP (Zrt-, Irt-like proteins) transporters of high Mn affinity are involved in Mn-specific hyperaccumulation in this plant, we isolated ZIP family transporter gene homologs and investigated their metal transport characteristics in yeast cells. An isolated Zip gene homolog, designated as CsZIP1, coded a protein of 475 amino acids in length and showed 65% or lower sequence identity with ZIPs of other herbal plants. Expression of this gene was induced in the callus of Mn-deficient C. sciadophylloides. However, its expression did not rescue the Mn transport ability of yeast lacking the Mn transporter gene smf1. CsZIP1 did not show Zn and Fe transport, but its expression increased Cd sensitivity and Ni tolerance of wild-type yeast.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.