Abstract
The following glycolysis genes have been obtained by complementation in the appropriate yeast mutant from a pool of yeast DNA in the multicopy plasmid YEp13: PGI 1 (phosphoglucose isomerase), TPI 1 (triose-P isomerase), PGK 1 (phosphoglycerate kinase), GPM 1 (phosphoglycerate mutase), PYK 1 (pyruvate kinase), and GCR 1 (glycolysis regulation). The glycolysis enzymes include some of the major proteins of yeast, and in the clones (other than GCR 1) their levels, for the particular one, are 5–10 times normal.
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