Abstract
Allatostatins (ASTs) are insect neuropeptides that were first identified as inhibitors of juvenile hormone biosynthesis by the corpora allata. There are three families of ASTs in insects, defined by their C-terminus conserved regions, one of which is FGLamide. Here we determine, for the first time in a hemipteran, the complete 1013 bp cDNA sequence encoding the Rhodnius prolixus FGLa/ASTs (Rhopr-FGLa/ASTs), and confirm the transcript size using northern blot. Phylogenetic analysis suggests that the Rhopr-FGLa/AST prepropeptide is most similar to the FGLa/AST precursors identified in Hymenoptera. Reverse-transcriptase PCR demonstrates that the Rhopr-FGLa/AST transcript is highly expressed in the central nervous system (CNS) in unfed fifth-instar R. prolixus, and is reduced in expression in CNS dissected from one day old blood-fed insects. Fluorescent in situ hybridization shows transcript expression in neurons in each ganglion of the CNS, but also in cells located on peripheral nerves. Rhopr-FGLa/ASTs dose-dependently inhibit contractions of the anterior midgut and hindgut, suggesting a role in feeding-related physiological events.
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