Cloning of Ten Peroxidase (POD) Genes from Tamarix Hispida and Characterization of their Responses to Abiotic Stress

Abstract

Plant peroxidases (PODs) have been ascribed a variety of biological functions, including hydrogen peroxide detoxification, lignin biosynthesis, hormonal signaling, and stress response. In the present study, ten POD genes, including three ascorbate peroxidases (class I PODs) and seven secretory peroxidases (class III PODs), were cloned from Tamarix hispida. The roles of the ten POD genes were addressed under different abiotic stress conditions, and gene expression profiles in roots, stems, and leaves were evaluated using real-time quantitative reverse-transcribed polymerase chain reaction. Our results showed that the relative abundance of the PODs was markedly different in roots, stems, and leaves, indicating that POD activity differs in these three organs. ThPOD1 and ThPOD8 were the most and least abundant, respectively, in all organs. The expression profiles in response to abiotic stresses were organ specific. All of the genes were highly induced by drought, salt, salt–alkaline, CdCl2, and abscisic acid (ABA) treatments in at least one organ. Five ThPOD genes were induced in roots, stems, and leaves under all of the studied stress conditions, indicating that they are closely associated with abiotic stress. Our results demonstrate that the ten plant peroxidases are all expressed in leaves, stems, and roots, that they are involved in different abiotic stress responses, and that they are controlled by an ABA-dependent stress signaling pathway.