Abstract
A specialized transducing phage ρ11spo0F, containing one of the stage 0 sporulation genes, spo0F, of Bacillus subtilis was constructed. Sporulation of spo0F mutant of B. subtilis was restored by lysogenization with ρ11spo0F, indicating that the mutant allele was recessive to the wild type. The physical map of ρ11spo0F genome was constructed by using restriction endonucleases BamHI and SalI and compared with that of ρ11wt genome. The results indicate that the spo0F gene resides on the SalI-B fragment of ρ11spo0F genome. It was also found that the 1.3×106 dalton EcoRI fragment of ρ11spo0F DNA contained the spo0F gene.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
