Cloning of Six Full-Length cDNAs Encoding Pig Cytochrome P450 Enzymes and Gene Expression of these Enzymes in the Liver and Kidney

Abstract

Six full-length cDNAs encoding pig cytochrome P450 (CYP) enzymes, CYP1A1, CYP2A19, CYP2B22, CYP2C33v4, CYP2C49, and CYP2E1, were isolated and sequenced. The cDNA sequences of pig CYP1A1, CYP2A19, CYP2B22, CYP2C49, and CYP2E1 showed high similarity to human CYP1A1 (85.4%), CYP2A13 (88.6%), CYP2B6 (81.1%), CYP2C18 (85.3%), and CYP2E1 (82.5%), respectively, and pig CYP2C33v4 cDNA showed high similarity to rat CYP2C23 (79.2%). Reverse transcription-polymerase chain reaction (RT-PCR) assays revealed hepatic gene expression of all these pig CYP enzymes: the order of expression was CYP2C33v4 and CYP2E1 > CYP2C49 > CYP1A1 and CYP2A19 > CYP2B22. In the kidney, the CYP2C33v4 gene was expressed at the same level as in the liver, but the CYP1A1, CYP2A19, and CYP2B22 genes were expressed at lower levels than in the liver. Little renal gene expression of CYP2C49 and CYP2E1 was observed. We revealed for the first time the full-length cDNA sequences encoding pig CYP1A1 and five CYP enzymes belonging to the CYP2 gene family, thus making it possible to examine the gene expression levels of these CYP enzymes in pig tissues by RT-PCR.