Cloning of POMC cDNA and the stress response in Coilia nasus

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 刀鲚POMC基因的cDNA克隆及其应激应答 DOI: 作者: 作者单位: 1. 农业部淡水渔业健康养殖重点实验室, 浙江省淡水水产遗传育种重点实验室, 浙江省淡水水产研究所, 浙江 湖州 313001;2. 中国水产科学研究院 淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081 作者简介: 杜富宽(1983-),男,助理研究员.E-mail:adublg@126.com 通讯作者: 中图分类号: S96 基金项目: 农业部淡水渔业健康养殖重点实验室开放课题（ZJK2014003）；中国水产科学研究院淡水渔业研究中心中央级公益性科研院所基本科研业务费专项资金（2015JBFR06）；江苏省科技支撑项目（BE2014307）. Cloning of POMC cDNA and the stress response in Coilia nasus Author: Affiliation: 1. Key Laboratory of Healthy and Freshwater Aquaculture, Ministry of Agriculture;Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture;Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为了研究刀鲚（POMC基因的cDNA全长。刀鲚基因的cDNA全长2030 bp，编码区699 bp，编码232个氨基酸。二级结构预测显示，刀鲚POMC包括信号肽（signal peptide）、N末端区（N-terminal region，NPP）、促肾上腺皮质激素结构域（adrenocorticotropic hormone，ACTH）、γ-脂解素（-EP）结构域。运用荧光定量的方法检测了该基因在刀鲚不同组织中的表达分布。结果显示，基因的表达量显著下降（<0.05），6 h的表达量恢复至应激前。基因在脂肪代谢及应激调控中发挥重要的功能，本研究结果可为该基因在刀鲚后续育种及应激调控工作提供重要基础。 Abstract:The estuarine tapertail anchovy () is widely distributed in the Yangtze River, the coastal waters of China and Korea, and the Ariake Sound of Japan. It is a commercially important species because of its nutritive value and popularity. However, excess fishing and changes in aquatic ecology have almost caused extinction of this species in the middle reaches of the Yangtze River. In recent years, several studies have been performed on pond breeding and artificial propagation, and larval rearing techniques have been established. Thus, the threat to is very sensitive to stress, which often results in death and huge economic losses. Little is known about the mechanism by which loading stress causes death in fish. Pro-opiomelanocortin (POMC) is synthesized in the corticotrophs and melanotrophs of the anterior and intermediate lobes of the pituitary, respectively, as well as in peptidergic neurons in the arcuate nucleus of the hypothalamus. It is post-translationally cleaved into peptide hormones, including adrenocorticotrophic hormone, -melanocyte stimulating hormone (MSH), N-POMC, and -LPH) in tissue- and cell dependent manners. These peptides exhibit different physiological functions, such as promoting ovarian maturation, lipolysis, inhibiting fat accumulation, regulating osmotic pressure, and reacting to stressors. We cloned the and determined that the full-length cDNA is 1212 bp long, with a 699 bp open reading frame that encoded a 232 amino acid protein. The POMC protein is predicted to contain the typical POMC domains of a signal peptide sequence, the N-terminal region, and the adrenocorticotrophic hormone, -EP domains. Gene expression patterns were detected in different tissues by quantitative reverse transcription-polymerasechain reaction. was expressed strongly in the brain and relatively highly in the gill, kidney, and testis, whereas it was expressed at low levels in the liver, spleen, intestine, head kidney, muscle, and ovary. <0.05) and was significantly up regulated 4 h and 6 h after transport ( expression returned to the control level before stress. The gene plays an important role regulating fat metabolism and stress. Our results provide important clues for breeding and stress control studies in . 参考文献 相似文献 引证文献