Cloning of novel chemokines using a signal sequence trap method.

Abstract

AbstractPrecursors of most secreted and cell surface molecules carry signal sequences at their amino termini. The method coined as signal sequence trap (1–3) takes advantage of the presence of N-terminal signal sequences in most precursor forms of secretory proteins and transmembrane proteins, which are necessary for the proper orientation of the N-terminal of mature forms inside endoplasmic reticulum and exocytotic vesicles. This method enables to selectively clone cDNA species encoding intercellular signal-transducing molecules without biologic assays. In this chapter, an efficient signal sequence trap method based on an Epstein-Barr virus shuttle vector is described (2).KeywordsSignal SequenceMicrocentrifuge TubeRaji CellResidual EthanolAbsolute EtOHThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.