Cloning of merA Gene from Methylotenera Mobilis for Mercury Biotransformation.

Abstract

Mercury (Hg) is one of the most toxic heavy metal and is extremely harmful for the environment. The permissible limit of mercury in industrial effluents is 0.001ppm, whereas there are various sites having very high levels of mercury contamination. In the present study, 10 different mercury (Hg) resistant bacterial strains were isolated from Ulhas Estuary, Mumbai (Hg concentration of 107ppm). All the strains were subsequently grown on higher concentration of mercuric chloride (HgCl2), one of the isolate (USP5) showed significant growth at high concentration of Hg (40ppm) and 16S rRNA gene sequencing revealed the identity of the bacterium as Methylotenera mobilis, (Accession no. KT714144). The mer operon was isolated and cloned in E.coli and checked for its ability to tolerate higher concentration of Hg. It has shown growth up to 70ppm of Hg, also presence of merA gene indicated its ability to detoxify Hg into less toxic volatile form. The atomic absorption spectrophotometry confirmed the ability of clone to efficiently detoxify 60-90% of the Hg (10-70ppm) within 48-72h. This clone can be used for effective volatilization of Hg from contaminated areas.