Cloning of invertebrate gonadotropin-releasing hormone receptor (GnRHR)-like gene in Yesso scallop, Patinopecten yessoensis

Abstract

Although the invGnRH peptide was characterized from the nerve ganglia in bivalves, its signaling mechanism is nevertheless unclear. The objective of this paper was to identify the invGnRHR cDNA from Yesso scallop as a first step for understanding of invGnRH in bivalve neuroendocrine system. We performed PCR cloning mediated with transcriptome survey and expression analysis with various tissues of the scallop. Our results showed that we identified an invGnRHR-like cDNAs from not only Yesso scallop Patinopecten yessoensis but also Pacific oyster Crassostrea gigas. In addition, we subsequently identified an adipokinetic hormone receptor (AKHR)-like and AKH-like cDNA pair from the scallop. Comparison of the tissue distributions of both receptor mRNAs suggested functional divergence of two homologous neuropeptides. In brief, py-GnRHR-like mRNA showed broad distribution in various tissues including the nerve ganglia, while py-AKHR-like mRNA was expressed in the nerve ganglia and restricted to some limited peripheral tissues. The findings suggested that both py-GnRH and py-AKH signals were utilized via their own receptors. qPCR assays revealed their receptor mRNA expression in the gonads during a maturation, showing that py-GnRHR-like mRNA in the pre-mature gonads was higher than other mature stages.