Cloning of IGF-I, IGF-II and IGF-IR cDNAs in Mullet (Mugil cephalus) and Grouper (Epinephelus coioides): Molecular Markers for Egg Quality in Marine Fish

Abstract

The authors would like to acknowledge the assistance of Hazra Thaggard and Dr. Mike Rimmer in the sample collection. This study was part of the John Allwright Fellowship of the Australian Centre for International Agricultural Research with the project collaboration of SEAFDEC/AQD granted to JG (2002-2004). A. Elizur, A. Anderson and N. Richardson provided concept for the work, J. Nocillado helped design primers for amplification of the target genes and assisted in the sample and tissue collection and J. Gonzaga performed gene cloning, mRNA expression assay and statistical analysis.