Abstract
Neuropeptide Y, peptide YY, and pancreatic polypeptide are homologous 36-amino acid peptides that differ from most other peptide transmitters by having a relatively rigid conformation in aqueous solutions, defined as the pancreatic polypeptide fold, and a critical C-terminal tyrosine amide. These peptides serve as gastrointestinal hormones and neurotransmitters. A cDNA encoding a novel G protein-coupled receptor activated by neuropeptide Y was cloned from Drosophila by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed early in embryogenesis. The cDNA encodes a protein of 449 amino acids with the characteristics of a G protein-coupled receptor and shares significant amino acid identity with mammalian tachykinin receptors. When expressed in Xenopus oocytes, the PR4 protein is activated by mammalian neuropeptides in the order: peptide YY greater than neuropeptide Y much greater than pancreatic polypeptide. Northern analysis showed that PR4 receptor is expressed at equivalent levels in adult Drosophila head and body and that the expression of the PR4 receptor is regulated during development. The molecular characterization of this receptor should lead to a better understanding of the functional role of this important family of hormone receptors in adult organisms and during development.
Highlights
Regulation of shown to affect memory and food intake (10,ll).Some of the cellular actions of Neuropeptide Y (NPY) are mediated through G proteins [25]
We report the isolation of a Neuropeptide Y, peptide YY, and pancreatic poly- cDNA clone that encodes a G protein-coupled receptor actipeptide are homologous 36-amino acid peptides that vated by NPY andPYY when expressed in Xenopus oocytes
A cDNA encoding a novel G protein-coupled receptor activated by neuropeptide Y was cloned from Drosophila by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed earlyin embryogenated from poly(A)+RNA samples using murine leukemia virus reverse transcriptase (Bethesda Research Laboratories)
Summary
Regulation of shown to affect memory and food intake (10,ll).Some of the cellular actions of NPY are mediated through G proteins [25]. G protein-coupled receptors that have seven transmembrane domains. We have identified a Drosophila tachykinin receptor whose expression coincides with the beginning of neural development during early stages of embryogenesis [16].
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