Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti

Guo-Hui Zhao,Zhao-Hui Yang,Chen-Xu Wu,Liang Jin,Xiao-Qiang Yu,Jian-Nan Liu,Xiao-Hua Hu,Xiao-Yan Jiang,Ling-Ling Zhang,Juan Wu,Khadija Batool,En-Jiong Huang,Xian-Hui Huang,Xiong Guan,Hong Chen

doi:10.1186/s13071-019-3560-2

Abstract

BackgroundBacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. However, the function of ABP in mosquito biocontrol is little known, especially in Aedes aegypti. Therefore, this study aimed to clarify the function of ABP in Ae. aegypti against Bti toxin.ResultsAedes aegypti ABP (GenBank: XM_001661856.2) was cloned, expressed and purified in this study. Far-western blotting and ELISA were also carried out to confirm the interaction between ABP and Cry11Aa. A bioassay of Cry11Aa was performed both in the presence and absence of ABP, which showed that the mortality of Ae. aegypti is increased with an increase in ABP.ConclusionsOur results suggest that ABP in Ae. aegypti can modulate the toxicity of Cry11Aa toxin to mosquitoes by binding to Bti toxin. This could not only enrich the mechanism of Bt toxin, but also provide more data for the biocontrol of this transmission vector.

Highlights

Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity
Cloning the ATP-binding proteins (ABP) gene The polymerase chain reaction (PCR) result showed that the full length ABP gene (GenBank: XM_001661856.2) has an open reading frame (ORF) of 915 bp encoding 304 amino acids (Fig. 1)
ABP is detected in many insects related to the process of the Bti toxin reaction, which is widely used in mosquito biocontrol

Summary

Introduction

Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. The function of ABP in mosquito biocontrol is little known, especially in Aedes aegypti. This study aimed to clarify the function of ABP in Ae. aegypti against Bti toxin. Mosquitoes, such as Anopheles, Aedes and Culex, are important infectious medium in transmitting various tropical diseases. Global health problems associated with mosquito-borne diseases put hundreds of millions of people at risk [1,2,3]. The attempt to control and early detection of these infections becomes a global public health issue [4]. Vector control is the main method for preventing the spread of these diseases [2, 6, 7]

Objectives

Methods

Results

Discussion

Conclusion