Abstract
A Lupinus luteus chloroplast clone bank was analysed for the presence of ndh genes using tobacco heterologous probes. Six ndh genes, ndhC, ndhK, ndhJ, ndhD, ndhE and ndhF, were identified. A BamHI clone 14, consisting of a 9 kb insert, was partially sequenced. A 1.9 kb long sequence was found to contain ndhC, ndhK and ndhJ genes (EMBL accession no. for ndhC is U27653, for ndhK U27654 and for ndhJ U85664). The reading frames of ndhC and ndhK genes, unlike those of other land plants, do not overlap. Northern blot hybridization as well as RT-PCR revealed that the three genes are co-transcribed. The transcripts were identified in the total RNA isolated from leaves and in the chloroplast fractions.
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