Abstract
Based on the sequences of various CMAH genes, our group previously cloned the pig CMAH gene (pcmah) that encodes the CMP-N-acetylneuraminic acid hydroxylase from pig tissues. In this study, we have cloned the complete pcmah gene by using rapid amplification of cDNA ends (RACE), and also isolated 5’ alternative transcription start sites and 3’ alternative splicing variants of pcmah. 5’RACE analysis revealed that pcmah has two alternative spliced forms, with two different initiation sites located in exon 0 and exon 1a of the gene expressed by pig small intestine. Also, 3’ RACE revealed that pcmah has three 3’ alternative splicing variants, pcmah variant 2 with nine exons and variant3 eight exons. Furthermore, the mRNA expression of the pcmah gene and its variants were analyzed in various pig tissues by reverse transcription polymerase chain reaction (RT-PCR).
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