Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura.

Abstract

Juvenile hormone (JH) is one of key insect hormones that regulate metamorphosis. Juvenile hormone diol kinase (JHDK) is an enzyme involved in JH metabolism and catalyzes JH diol to form a polar end product, JH diol phosphate that has no JH activity. In this study, a JHDK cDNA was cloned from Spodoptera litura and the structure and expression of the gene was characterized. The cDNA was 714 base pairs in length and encoded a protein of 183 amino acids with a molecular mass of 21 kDa and a pI of 4.55. Based on the structure three putative calcium binding motifs and GTP-binding motifs were predicted in the protein. Modeling of the 3-D structure showed that the protein consisted of eight α-helixes linked with loops, with no β-sheets. The gene was expressed in the epidermis, fat body and midgut of 5th and 6th instar larvae. The expression level in the epidermis was lower than in the fat body and midgut. The gene was expressed at higher levels at the early stages than in the later stages of 5th and 6th instar midgut and fat body. The results suggest that this gene may be involved in the regulation of the JH titer in larvae of S. litura. This article is protected by copyright. All rights reserved.