Abstract
Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer’s disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest. These data suggest as yet uncharacterized roles for ApoCI during early vertebrate embryogenesis.
Highlights
Accepted: December 10, 2017Published: January 18, 2018
The gene encoding the 3–2 transcript maps to Xenopus laevis chromosome 7L (XLA7L) (X. laevis 9.2 on Jbrowse); there is no apoCI-like gene on XLA7S, suggesting that there is no second identifiable apoCIlike allogene in the Xenopus laevis genome
Xenopus apoCI expression is enriched in the dorsal ectoderm
Summary
Data Availability Statement: All relevant data are within the paper and its Supporting Information files. ApoCI inhibits hepatic remnant clearance by preventing ApoE-mediated binding and uptake of VLDLs by LDL receptor Related Protein (LRP), VLDL receptor (VLDLr) and LDL receptor (LDLr), and by inhibition of lipoprotein lipase activity [4,5,6,7]. We describe the cloning and expression analysis of apoCI in embryos of the frog Xenopus laevis; our studies suggest important and perhaps conserved roles for ApoCI during gastrulation and subsequent patterning of the vertebrate ectoderm
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