Cloning and sequencing of the gene encoding the plasma membrane H +-ATPase from an acidophilic red alga, Cyanidium caldarium

Abstract

A cDNA containing an open reading frame encoding the putative plasma membrane H +-ATPase in an acidophilic red alga, Cyanidium caldarium, was cloned and sequenced by means of PCR and Southern hybridization based on homologous sequences of P-type ATPases found in other organisms. The cloned cDNA is 3300 bp in length, containing a 2865 bp open reading frame encoding a polypeptide of 955 amino acids which has a predicted molecular mass of 105 371. The deduced amino acid sequence was found to be more homologous to those of P-type H +-ATPases from higher plants than that from the green alga Dunaliella bioculata.©1997 Elsevier Science B.V. All rights reserved.