Abstract
Specific amplification by PCR of gamma-gliadin genes from Triticum turgidum conv. durum cv. Langdon was obtained using high temperature for DNA-primers annealing. Three amplified bands were obtained and one of these was cloned and sequenced. Comparison of this nucleotide sequence with those of published gamma-gliadin and gamma-hordein genes revealed a high sequence homology. In particular, the comparison with pW1020 clone isolated from T. aestivum showed that the sequences derived from two genotypes differed by obly two nucleotides. The advantages offered by this approach for rapid gene isolation from different genotypes and its importance in molecular genetic analysis of wheat storage proteins are discussed.
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