Abstract
A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, TK420, defective in K + transport genes ( kdpABC, trkD, trkA). The cloned Vibrio gene allowed TK420 to grow in a synthetic medium containing less than 10 mM K + and concomitantly led to an increase in K + uptake activity. The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 458 amino acid sequence and molecular mass of 50 122 Da. This gene has 71% homology to trkA gene at the DNA level from E. coli and the deduced amino acid sequence is 79% identical with E. coli TrkA, implying that V. alginolyticus has a trkA-like gene as a component of K + transport systems.
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