Abstract
Northern blot analysis showed that a metallothionein gene,ricMT, is expressed strongly in the stem of rice with an expression level that could be more than 100-fold stronger than in leaf blades. The results suggest that the 5′ upstream region flanking the coding sequence of thericMT may contain a fairly strong promoter. To elucidate its regulation and promoter structure, the genomic clones ofricMT were screened out from a rice genomic library and a fragment of about 4 084 bp was sequenced. The fragment included a 5′ upstream region of ca. 2 970 bp, a transcription region of ca. 690 bp and a 3′ downstream region of ca. 420 bp. Computer analysis of the sequence homology showed that the 5′ upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG. The results will promote further understanding of the mechanisms of gene regulation and metal response of plant metallothionein proteins.
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