Abstract
To analyze the sequence variation in the mitochondrial cytochrome c oxidase subunit 1(cox1) gene among Chabertia ovina(C. ovina) isolates from goat in Shaanxi province, China and to study its phylogenetic relationships with other nematodes using the cox1 gene sequences, the partial cox1(pcox1) was amplified from individual C. ovina samples by PCR. The amplified pcox1 sequences were aligned using the ClustalX 1.81 and MP tree was constructed using the software PAUP 4.0. The lengths of all the pcox1 sequences were 393 bp. Phylogenetic analyses showed that all the C.ovina samples from Shaanxi province were clustered in the same clade. Sequences variations in pcox1within C. ovina were limited(0.5%–2.0%), while inter-species differences were significant(9.4%–18.8%). Thus, the pcox1 sequences can be used as a genetic marker for identification and population genetic studies of nematodes.
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