Abstract
AGAMOUS (AG), a member of MADS-box gene family, is a key gene related to regulation of flower development in plants. The AG homologous gene GBM5 (Accession No.: AY114304.1) was obtained from the transcriptome of Ginkgo biloba . Primers were designed according to the obtained GBM5 gene sequence, and the full-length cDNA sequence of GBM5 gene with 732 bp in size and 243 amino acids in coding product can be synthetized by designing primers. The comparative analysis of evolutionary tree and amino acid sequence showed that ginkgo GBM5 was closely related to cycads AG and black spruce AG . qPCR analysis showed that the expression of GBM5 in different tissues of Ginkgo biloba was different at different periods, the expression levels of different tissues are as follows: Female flower>Male flower>Primordia>Foliage; Its expression is high in the undifferentiated stage of flower bud, the expression patterns were: April leaf>May leaf>September leaf, April primordia>May primordia>September primordia.
Highlights
MADS-box gene family is a group of transcription factors that regulate plant growth and development, especially in flower meristem development, control flowering time and flower organ development (Yao et al, 2018)
The comparative analysis of evolutionary tree and amino acid sequence showed that ginkgo GBM5 was closely related to cycads AG and black spruce AG. qPCR analysis showed that the expression of GBM5 in different tissues of Ginkgo biloba was different at different periods, the expression levels of different tissues are as follows: Female flower>Male flower>Primordia>Foliage; Its expression is high in the undifferentiated stage of flower bud, the expression patterns were: April leaf>May leaf>September leaf, April primordia>May primordia>September primordia
A specific primer was designed according to the measured ginkgo transcriptome data, and 732 bp GBM5 gene was cloned from Ginkgo biloba by RT-PCR technology
Summary
MADS-box gene family is a group of transcription factors that regulate plant growth and development, especially in flower meristem development, control flowering time and flower organ development (Yao et al, 2018). The N-terminal of the proteins encoded by them all contain MADS-box conserved domain composed of 50~60 amino acids (Liu et al, 2010). K region is a unique sequence of MADS-box transcription factors in plants, with about 70 amino acid residues (Zhang et al, 2013). GbCO gene was first cloned from Ginkgo biloba in 2010, encoding a protein composed of 373 amino acids It contains two B-box zinc finger molds (Zhang, 2002). As a ubiquitous transcription factor, FLC gene in MADS-box gene family plays an important role in the vernalization process of plants (Hong and Cao, 2002). The expression level of MADS-box protein is closely related to the flowering time of plants. Several flowering regulation pathways are interrelated and jointly regulate the flowering of higher plants
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