Cloning and Primary Functional Analysis of &lt;i&gt;PeGRF1&lt;/i&gt; in &lt;i&gt;Phyllostachys edulis&lt;/i&gt;

Yongfeng Lou,Xiaochen Song,Huayu Sun,Zhimin Gao

doi:10.5376/mpb.2020.11.0030

Abstract

To explore the role of Growth regulating-factor (GRF) in the growth of bamboo shoots, one GRF gene was isolated from moso bamboo ( Phyllostachys edulis ) by RT-PCR. Bioinformatics methods were used for gene sequence analysis, and the expression pattern of the GRF gene in bamboo shoots at different development stages was analyzed by qRT-PCR. Simultaneously, ectopic expression in Arabidopsis was conducted to validate the gene function. The result showed that the isolated gene from moso bamboo was 1 164 bp, encoding 387 amino acids, which was named as PeGRF1 . Protein sequence analysis showed that PeGRF1 had the complete typical domains (WRC and QLQ) of GRF family. The phylogenetic analysis demonstrated that PeGRF1 were clustered closer to the GRFs of monocotyledonous plants such as Oryza sativa , indicating they had close relationship. PeGRF1 expressed predominantly in young bamboo shoots. Meanwhile, the expression level of PeGRF1 was significantly higher during rapid growth stage than that during slow growth stage of bamboo shoots, and the expression level of PeGRF1 in the upper and middle of bamboo shoots was significantly higher than that in the base. Overexpression of PeGRF1 could increase the plant height of transgenic Arabidopsis . Taken together, our results demonstrated that PeGRF1 was involved in development of bamboo shoots, which provided references for elucidating the biological functions of GRF genes in bamboo.

Highlights

The Growth regulating factor (GRF) is a plant-specific transcription factor, and that playing an important role in regulating plant growth and development (Omidbakhshfard et al, 2015; Yuan et al, 2017; He et al, 2018)
Our results demonstrated that PeGRF1 was involved in development of bamboo shoots, which provided references for elucidating the biological functions of Growth regulating-factor (GRF) genes in bamboo
1.1 Molecular characterization of PeGRF1 in moso bamboo A cDNA fragment of 1 200 bp was obtained from moso bamboo (Ph. edulis) leaves using the primers, which was consistent with the expected size (Figure 1)

Summary

Introduction

The Growth regulating factor (GRF) is a plant-specific transcription factor, and that playing an important role in regulating plant growth and development (Omidbakhshfard et al, 2015; Yuan et al, 2017; He et al, 2018). Bioinformatics methods were used for gene sequence analysis, and the expression pattern of the GRF gene in bamboo shoots at different development stages was analyzed by qRT-PCR.

Results

Conclusion