Abstract
Objective To obtain the chicken anemia virus(CAV) vp3 gene from Zhejiang region chicken and analyze its variation.Methods The specific primers were designed according to the published sequences in GenBank.By PCR,7 clones of CAV vp3 gene were obtained from CAV-infected chicken.Results 16 CAV-positive chicken serum were detected in 40 samples,and the positive rate was 40.0％.Sequencing results showed that vp3 gene consisted of 366 bp,coded 121 amino acids,and shared 97.0％-100.0％ nucleotide homology and 95.0％-100.0％ protein homology with that of the other strains submitted to GenBank.Conclusions CAV vp3 gene is obtained by PCR,vp3 gene and VP3 protein are very conservative. Key words: Chicken anemia virus; vp3 gene; Cloning; Molecular characteristics
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