Abstract
African swine fever virus DNA (about 170 kbp) was cleaved with the restriction endonuclease Eco RI and the resulting fragments, before or after cleavage with the endonuclease Sal I, were cloned in plasmid or phage vectors. The two terminal Eco RI fragments were cloned after removal of the crosslinks with nuclease S1 and addition of Eco RI linkers to the fragment ends. The order of the restriction fragments produced by the restriction endonucleases Sal I, Eco RI, Kpn I, Pvu I and Sma I was established by identifying the crosslinked Eco RI and Sal I terminal fragments and overlapping fragments.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
